Postlabelling methods for detection of DNA adducts
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Postlabelling methods for detection of DNA adducts

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Published by International Agency for Research on Cancer in Lyon .
Written in English


  • Carcinogenesis -- Research -- Methodology -- Congresses.,
  • DNA-ligand interactions -- Congresses.,
  • Phosphorus -- Isotopes -- Congresses.,
  • DNA damage -- Congresses.,
  • Biochemical markers -- Congresses.,
  • Biological Markers -- congresses,
  • Environmental Monitoring -- methods -- congresses,
  • DNA Damage -- congresses,
  • Autoradiography -- methods -- congresses,
  • Phosphorus Radioisotopes -- congresses,
  • DNA -- analysis -- congresses,
  • Carcinogens -- analysis -- congresses

Book details:

Edition Notes

Statementedited by D.H. Phillips, M. Castegnaro and H. Bartsch.
SeriesIARC scientific publications,, no. 124
ContributionsPhillips, D. H., Castegnaro, M. 1944-, Bartsch, H., International Agency for Research on Cancer., United States. Environmental Protection Agency., Germany. Ministry of Health.
LC ClassificationsRC268.5 .P67 1993
The Physical Object
Paginationxii, 392 p. :
Number of Pages392
ID Numbers
Open LibraryOL1158129M
ISBN 109283221249
LC Control Number94135660

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In experimental animal studies, the quantitation of DNA adducts has usually required the use of highly radioactive chemical carcinogens. However, a major breakthrough in detection methods occurred in the early s with the development of the 32P-postlabelling technique. Today, at least 60 laboratories have employed and customized these methods.   The detection and characterisation of DNA adducts can provide mechanistic information on mode of action for genotoxic chemicals and in this context is Cited by:   This is the first book devoted to postlabelling methods, an important new analytical methodology. Postlabelling methods have a wide range of applications: these include studies of pathways of metabolic activation of chemical carcinogens, studies of oxidative damage to DNA, monitoring occupational exposure to carcinogens, the association between DNA adducts and disease, the Pages: Figure 1 shows typical DNA adducts and figure 2 illustrates adduct levels measured in mussels (Mytilus galloprovincialis) of the Venice lagoon over tissue samples (gills or digestive glands) from at least 5 mussels were tested in the 32 P-postlabelling assay, as described previously (Venier and Canova, ).More recently, improvements related to DNA purification (xg Cited by: 3.

  Introduction. The 32 P-postlabelling assay is an ultrasensitive method for detecting carcinogen–DNA adducts that is applicable to a very wide range of DNA lesions (Gupta et al., ; Beach and Gupta, ; Randerath and Randerath, ; Phillips, ).It has been applied to a large number of studies in which human exposure to genotoxic agents has been by: 32 P-Postlabelling also enables the long-term persistence of carcinogen–DNA adducts to be monitored. Thus 7,dimethylbenz-(a)anthracene adducts have been detected in mouse skin DNA 10 months after topical application of the compound (Randerath et al., ), and safrole–DNA adducts persist in mouse liver at least 4 months after intraperitoneal administration (Randerath et al., ).Author: D.H. Phillips. Harvey, J. S., and Parry, J. M. () Application of the 32 P-postlabelling assay for the detection of DNA adducts: False positives and artefacts and their implications for environmental biomonitoring, Aquatic Toxicol – CrossRef Google ScholarCited by: 7. 32P-postlabelling for the Sensitive Detection of DNA Adducts Article (PDF Available) in Methods in molecular biology (Clifton, N.J.) January with 64 Reads How we measure 'reads'.

Comparison of DNA adduct detection between two enhancement methods of the 32P-postlabelling assay in rat lung cells. Whong WZ(1), Stewart JD, Ong T. Author information: (1)Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV Cited by: Application of the 32P-postlabelling technique to detect DNA-adducts by cisplatin and methylating agents. / Mustonen, R.; Hemminki, K. In: Progress in clinical and biological research, Vol. C, , p. Research output: Contribution to journal › ArticleCited by: 3. Our results show that DNA adducts of MA can be measured in humans using 32P-postlabelling in combination with nuclease P1 and reversed-phase HPLC as adduct enrichment procedures, and further Author: John Mclaren Howard. Horvath E, Pongracz K, Rappaport S, Bodell WJ. 32P-post-labeling detection of DNA adducts in mononuclear cells of workers occupationally exposed to styrene. Carcinogenesis. Jul; 15 (7)– Savela K, Hemminki K. DNA adducts in lymphocytes and granulocytes of smokers and nonsmokers detected by the 32P-postlabelling by: